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sc 100859  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology sc 100859
    Sc 100859, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sc+100859/sorcin+Antibody/pm37442828-65-119-120
    Average 91 stars, based on 22 article reviews
    sc 100859 - by Bioz Stars, 2026-09
    91/100 stars

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    Western Blot:

    Article Title: Sorcin promotes migration in cancer and regulates the EGF-dependent EGFR signaling pathways.
    Article Snippet: Lysates were sonicated for 15 s, centrifuged at 12,000 × rpm for 10 min to remove cell debris and then quantified in protein content with Bradford colorimetric assay (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. .. Western blotting was performed loading 30 μg of lysates and using the following primary antibodies: rabbit monoclonal EGFR (1:1000 in 5%BSA-T-TBS solution) (D38B1, #4267, Cell Signaling), rabbit monoclonal Phospho-EGF Receptor (1:1000 in 5%BSA-T-TBS solution) (Tyr1068, #3777 Cell Signaling, Danvers, MA, USA), rabbit monoclonal p44/42 MAPK (Erk1/2) (1:1000 in 5%BSA-T-TBS solution) (137F5, #4695 Cell Signaling, Danvers, MA, USA), rabbit monoclonal Phospho-ERK1/ERK2 (P44/ P42 MAPK) (1:1000 in 5%BSA-T-TBS solution) (T202/ Y204 # MAB-94112 Immunological Science, Italy), rabbit polyclonal CHD2 (1:1000 in 5%BSA-T-TBS solution) (#ab182013, Abcam), mouse monoclonal SLUG (A-7) (1:200 in 5%BSA-T-TBS solution) (#sc-166476 Santa Cruz Biotechnology, Heidelberg, Germany), mouse monoclonal SNAI1 (G-7) (1:200 in 5%BSA-T-TBS solution) (#sc-271977 Santa Cruz Biotechnology, Heidelberg, Germany), mouse monoclonal Sorcin (39-M) (1:200 in 5%BSA-T-TBS solution) (#sc-100859 Santa Cruz Biotechnology, Heidelberg, Germany), mouse monoclonal GAPDH (1:2000 in 5%BSA-T-TBS solution) (#TA802519, OriGene Technologies, Rockville, USA), mouse monoclonal α-Tubulin B-5–1-2 (1:3000 in 5%BSA-T-TBS solution) (#T5168, Sigma-Aldrich, Milan, Italy). .. As secondary antibodies were used goat anti-mouse (1:10,000 in 5% milk-TTBS solution) and anti-rabbit (1:5000 in 5% milk-T-TBS solution or 5%BSA-T-TBS solution for phosphorylated protein) conjugated to horseradish peroxidase (Bethyl Laboratories, Montgomery, TX, USA).



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    sc 100859  (Santa Cruz Biotechnology)
    91
    Santa Cruz Biotechnology sc 100859
    Sc 100859, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sc+100859/sorcin+Antibody/pm37442828-65-119-120
    Average 91 stars, based on 1 article reviews
    sc 100859 - by Bioz Stars, 2026-09
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    mouse monoclonal sorcin 39 m  (Santa Cruz Biotechnology)
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    Fig. 6 Role of <t>Sorcin</t> in cell migration after EGF and IGF treatment. Wound-healing assay in H1299 cells cultured in serum-free medium (SF) or in the presence of EGF and IGF, in control experiments (siNC) and upon silencing of Sorcin (siSRI). Images were acquired at 0 h and 24 h after each single treatment. Quantification of wound-healing assay calculated as percentage of wound closure: ((At0 – At1)/ At0) × 100), where At0 is the initial wound area and At1 is the wound area n hours after the initial scratch. Error bars indicate means ± SEM. **p < 0.01, ***p < 0.001 and ****p < 0.0001 as determined by Student’s t test (n = 3). Scale bars, 200 µm
    Mouse Monoclonal Sorcin 39 M, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sorcin  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology sorcin
    Fig. 6 Role of <t>Sorcin</t> in cell migration after EGF and IGF treatment. Wound-healing assay in H1299 cells cultured in serum-free medium (SF) or in the presence of EGF and IGF, in control experiments (siNC) and upon silencing of Sorcin (siSRI). Images were acquired at 0 h and 24 h after each single treatment. Quantification of wound-healing assay calculated as percentage of wound closure: ((At0 – At1)/ At0) × 100), where At0 is the initial wound area and At1 is the wound area n hours after the initial scratch. Error bars indicate means ± SEM. **p < 0.01, ***p < 0.001 and ****p < 0.0001 as determined by Student’s t test (n = 3). Scale bars, 200 µm
    Sorcin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sc+100859/sorcin+Antibody/pm35661618-90-28-29
    Average 91 stars, based on 1 article reviews
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    sorcin antibody  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology sorcin antibody
    Fig. 6 Role of <t>Sorcin</t> in cell migration after EGF and IGF treatment. Wound-healing assay in H1299 cells cultured in serum-free medium (SF) or in the presence of EGF and IGF, in control experiments (siNC) and upon silencing of Sorcin (siSRI). Images were acquired at 0 h and 24 h after each single treatment. Quantification of wound-healing assay calculated as percentage of wound closure: ((At0 – At1)/ At0) × 100), where At0 is the initial wound area and At1 is the wound area n hours after the initial scratch. Error bars indicate means ± SEM. **p < 0.01, ***p < 0.001 and ****p < 0.0001 as determined by Student’s t test (n = 3). Scale bars, 200 µm
    Sorcin Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sc+100859/sorcin+Antibody/10__1530_slash_jme___17___0153-59-21-24
    Average 91 stars, based on 1 article reviews
    sorcin antibody - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier

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    Fig. 6 Role of Sorcin in cell migration after EGF and IGF treatment. Wound-healing assay in H1299 cells cultured in serum-free medium (SF) or in the presence of EGF and IGF, in control experiments (siNC) and upon silencing of Sorcin (siSRI). Images were acquired at 0 h and 24 h after each single treatment. Quantification of wound-healing assay calculated as percentage of wound closure: ((At0 – At1)/ At0) × 100), where At0 is the initial wound area and At1 is the wound area n hours after the initial scratch. Error bars indicate means ± SEM. **p < 0.01, ***p < 0.001 and ****p < 0.0001 as determined by Student’s t test (n = 3). Scale bars, 200 µm

    Journal: Cellular and molecular life sciences : CMLS

    Article Title: Sorcin promotes migration in cancer and regulates the EGF-dependent EGFR signaling pathways.

    doi: 10.1007/s00018-023-04850-4

    Figure Lengend Snippet: Fig. 6 Role of Sorcin in cell migration after EGF and IGF treatment. Wound-healing assay in H1299 cells cultured in serum-free medium (SF) or in the presence of EGF and IGF, in control experiments (siNC) and upon silencing of Sorcin (siSRI). Images were acquired at 0 h and 24 h after each single treatment. Quantification of wound-healing assay calculated as percentage of wound closure: ((At0 – At1)/ At0) × 100), where At0 is the initial wound area and At1 is the wound area n hours after the initial scratch. Error bars indicate means ± SEM. **p < 0.01, ***p < 0.001 and ****p < 0.0001 as determined by Student’s t test (n = 3). Scale bars, 200 µm

    Article Snippet: Western blotting was performed loading 30 μg of lysates and using the following primary antibodies: rabbit monoclonal EGFR (1:1000 in 5%BSA-T-TBS solution) (D38B1, #4267, Cell Signaling), rabbit monoclonal Phospho-EGF Receptor (1:1000 in 5%BSA-T-TBS solution) (Tyr1068, #3777 Cell Signaling, Danvers, MA, USA), rabbit monoclonal p44/42 MAPK (Erk1/2) (1:1000 in 5%BSA-T-TBS solution) (137F5, #4695 Cell Signaling, Danvers, MA, USA), rabbit monoclonal Phospho-ERK1/ERK2 (P44/ P42 MAPK) (1:1000 in 5%BSA-T-TBS solution) (T202/ Y204 # MAB-94112 Immunological Science, Italy), rabbit polyclonal CHD2 (1:1000 in 5%BSA-T-TBS solution) (#ab182013, Abcam), mouse monoclonal SLUG (A-7) (1:200 in 5%BSA-T-TBS solution) (#sc-166476 Santa Cruz Biotechnology, Heidelberg, Germany), mouse monoclonal SNAI1 (G-7) (1:200 in 5%BSA-T-TBS solution) (#sc-271977 Santa Cruz Biotechnology, Heidelberg, Germany), mouse monoclonal Sorcin (39-M) (1:200 in 5%BSA-T-TBS solution) (#sc-100859 Santa Cruz Biotechnology, Heidelberg, Germany), mouse monoclonal GAPDH (1:2000 in 5%BSA-T-TBS solution) (#TA802519, OriGene Technologies, Rockville, USA), mouse monoclonal α-Tubulin B-5–1-2 (1:3000 in 5%BSA-T-TBS solution) (#T5168, Sigma-Aldrich, Milan, Italy).

    Techniques: Migration, Wound Healing Assay, Cell Culture, Control